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C2+
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Laser unit
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LU-N3 : installed laser 405 nm, 488 nm, and 561 nm, Cannot be used with C2-DUS spectral detector
LU-N4/N4S : installed laser 405 nm, 488 nm, 561 nm, and 640 nm, Use LU-N4S when using C2-DUS spectral detector
LU-NV series : mountable laser 405 nm, 445 nm, 458 nm, 488 nm, 514 nm, 532 nm, 561 nm, 594 nm, 640 nm and 647nm
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Standard detector
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Wavelength : 400-750 nm, Detector : 3PMTs, Filter cube: 2 filter cubes
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Diascopic detector(option)
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Wavelength : 400-700nm, Detector: 1 PMT
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Scan head
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C2+/C2si+ scan head: when configuring with C2-DU3 standard fluorescence detector
Scanner: Galvano scanner, Pixel size: 2048 x 2048
Scanning speed: Standard mode; 2 fps (512 x 512 pixels, bi-direction), 17 fps(512 x 32 pixels, bi-direction), Zoom: 1-1000x
Fast mode; 8 fps (512 x 512 pixels, bi-direction), 100 fps (512 x 32 pixels, bi-direction)*1 , Zoom: 8-1000x
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C2si+ scan head: when configuring with C2-DUS spectral detector
Scanner: Galvano scanner, Pixel size: max. 1024 x 1024 pixels
Scanning speed: 0.5 fps (512 x 512 pixels, single direction)
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Scanning mode
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X-Y, XY rotation, zoom, XYZ, time lapse, X-Z, stimulation, multipoint, image stitching (large image)
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Pinhole
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Circular shape, 6 size
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Spectral detector(option)*2
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C2-DUS spectral detector unit
Number of channels: 32, Wavelength detection range: 400-750 nm, Spectral image acquisition speed: 2 fps (256 x 256 pixels)
Maximum pixel size: 1024 x 1024 (Spectral mode/Virtual filter mode), Wavelength resolution: 2.5 / 5.0 / 10.0 nm, Wavelength range variable in 0.25 nm steps
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C2-DUVB GaAsP detector unit
Number of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT (C2-DUVB-OP) with a user-defined dichroic mirror and barrier filter, Wavelength detection range: 400 – 720 nm, narrowest: 10nm, broadest: 320 nm, maximum pixel size: 2048 x 2048 (CB mode/VB mode), Wavelength resolution: 10nm, Wavelength range variable in 1 nm steps
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FOV
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Square inscribed in a Φ18mm circle
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Image bit depth
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12 bits
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Compatible microscopes
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Inverted microscope ECLIPSE Ti-E/Ti-U, Upright microscope ECLIPSE Ni-E (focusing nosepiece type/focusing stage type)/Ni-U, Fixed stage microscope ECLIPSE FN1, Multi-purpose zoom microscope AZ100
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Z step
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Ti-E: 0.025 um, FN1 stepping motor: 0.05 um, Ni-E: 0.025 um
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Software
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Software: NIS-Elements C
Display/image processing/analysis
2D/3D/4D analysis, time-lapse analysis, 3D volume rendering/orthogonal, spatial filters, image stitching, multipoint time-lapse, spectral unmixing, real-time unmixing, virtual filters, deconvolution, AVI image file output
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Application: FRAP,FLIP, FRET, photoactivation, colocalization, three-dimensional time-lapse imaging, multipoint time-lapse imaging
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Control Computer
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OS: Microsoft Windows 7 Professional 64 bit SP1, CPU: Intel Xeon E5-1630v3 (3.70 GHz/10 MB/2133 MHz/Quad Core) or higher, Memory: 4 GB, Hard disk: Serial ATA 6 Gb/s (7,200 rpm) 146 GB or higher, Data transfer: LAN, Network interface: Gigabit Ethernet, Monitor: 1600 x 1200 or highter resolution, dual monitor, Configuration is recommended
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Dimensions and weight
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C2+/C2si+ scan head 145 (W) x 236.5 (H) x 70 (D) mm, Approx. 2 kg
Controller 225 (W) x 404 (H) x 382 (D) mm, Approx. 12 kg
Standard detector 225(W) x 154 (H) x 224 (D) mm, Approx. 5kg
Spectral detector C2-DUS: 289 (W) x 323 (H) x 593.5 (D) mm, Approx. 23 kg,
C2-DUVB: 360 (W) x 91 (H) x 595.5 (D) mm, Approx. 9 kg
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*1 The described frame rate is NOT available with Rotation, CROP, ROI, Spectral imaging and Stimulation.
*2 Only available with C2si+ scan head.
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