Nikon C2+ 共軛焦顯微鏡

Nikon C2+
Nikon 第二代個人實驗室使用的雷射共軛焦顯微鏡,是市場上最精巧的掃瞄器,不僅合使用者空間運用,並擁有卓越的光學系統,與多功能性軟硬體設計,一舉將雷射掃瞄速度提升二倍;而高速galvano scanners,更可將速度拉升至100 fps。另外,新增的GaAsP Spectral detector磷砷化鎵光電倍增管,敏感度更高,是個人實驗室的最佳選擇。
 
Nikon C2+

高速擷取高畫質影像:

C2+利用pixel shift correction mechanism,修正傳統confocal system在Fast bi-directional scanning掃瞄模式下的像素位移,達到高速擷取目的。
 
Nikon C2+

直觀的操作介面:

搭配NIS-Elements影像操控軟體,可直觀操作Nikon顯微鏡與第三方硬體,協助完成各式各樣的實驗需求;因此不論是初學,亦或高手級共軛焦顯微鏡使用者,皆可快速上手。
Nikon C2+ Nikon C2+

影像品質-堅若鑽石:

  • 高效率掃瞄頭搭配訊號感測器

  • 高清晰度穿透式DIC影像

DIC image      Overlay of DIC and fluorescence images
  • 運用特殊奈米鍍膜技術Nano Crystal Coat technology,光線穿透率大大提升

 

強化型光譜影像偵測器:

  • C2-DUS Spectral Detector Unit : 32支可調校PMT感測器,一次掃瞄可涵蓋320nm波長範圍的訊號,波長解析度高達2.5nm。

 
  • C2-DUVB GaAsP Detector Unit

新型磷砷化鎵偵測器首次裝載於C2+系列,提供更高感測能力,可調式收光感測,讓使用者自定收光範圍,完成共軛焦光譜成像。 

 物鏡優化—λS與TIRF系列

  • λS 系列物鏡:運用Nikon獨有的奈米晶粒塗層技術,有效增加光線的穿透率。

  • TIRF系列物鏡:N.A.值高達1.49,

CFI Apochromat TIRF 60x oil / 1.49 (left)
CFI Apochromat TIRF 100x oil / 1.49 (right)

Laser Unit雷射模組:

  • LU-N4/N4S 4-laser unit, LU-N3 3-laser unit

Wavelength波長
Laser type 雷射型式
Output power光纖輸出能量
405nm
LD
15mW
488nm
LD*
15mW*
561nm
DPSS
15mW
640nm (for LU-N4/N4S)
LD
15mW
*  LU-N4S的488nm雷射配備DPSS laser,光纖輸出能量為8mW.
  • LU-NV Series:最多可配置8支不同波長雷射,並經由7個光纖輸出,提供最大的運用彈性。

Wavelength波長
Laser type 雷射型式
Output power光纖輸出能量
405nm
LD
20mW
455nm
LD
20mW
458nm
DPSS
18mW
488nm
DPSS
20mW, 45mW, 70mW
514nm
DPSS
20mW, 40mW
532nm
DPSS
40mW
561nm
DPSS
20mW, 45mW, 70mW
594nm
DPSS
20mW
640nm
LD
20mW, 40mW
647nm
Fiber
125mW

★Nikon C2+ specifications規格表:

C2+
Laser unit
LU-N3 : installed laser 405 nm, 488 nm, and 561 nm, Cannot be used with C2-DUS spectral detector
LU-N4/N4S : installed laser 405 nm, 488 nm, 561 nm, and 640 nm, Use LU-N4S when using C2-DUS spectral detector
LU-NV series : mountable laser 405 nm, 445 nm, 458 nm, 488 nm, 514 nm, 532 nm, 561 nm, 594 nm, 640 nm and 647nm
Standard detector
Wavelength : 400-750 nm, Detector : 3PMTs, Filter cube: 2 filter cubes
Diascopic detector(option)
Wavelength : 400-700nm, Detector: 1 PMT
Scan head
C2+/C2si+ scan head: when configuring with C2-DU3 standard fluorescence detector
Scanner: Galvano scanner, Pixel size: 2048 x 2048
Scanning speed: Standard mode; 2 fps (512 x 512 pixels, bi-direction), 17 fps(512 x 32 pixels, bi-direction), Zoom: 1-1000x
Fast mode; 8 fps (512 x 512 pixels, bi-direction), 100 fps (512 x 32 pixels, bi-direction)*1 , Zoom: 8-1000x
C2si+ scan head: when configuring with C2-DUS spectral detector
Scanner: Galvano scanner, Pixel size: max. 1024 x 1024 pixels
Scanning speed: 0.5 fps (512 x 512 pixels, single direction)
Scanning mode
X-Y, XY rotation, zoom, XYZ, time lapse, X-Z, stimulation, multipoint, image stitching (large image)
Pinhole
Circular shape, 6 size
Spectral detector(option)*2
C2-DUS spectral detector unit
Number of channels: 32, Wavelength detection range: 400-750 nm, Spectral image acquisition speed: 2 fps (256 x 256 pixels)
Maximum pixel size: 1024 x 1024 (Spectral mode/Virtual filter mode), Wavelength resolution: 2.5 / 5.0 / 10.0 nm, Wavelength range variable in 0.25 nm steps
C2-DUVB GaAsP detector unit
Number of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT (C2-DUVB-OP) with a user-defined dichroic mirror and barrier filter, Wavelength detection range: 400 – 720 nm, narrowest: 10nm, broadest: 320 nm, maximum pixel size: 2048 x 2048 (CB mode/VB mode), Wavelength resolution: 10nm, Wavelength range variable in 1 nm steps
FOV
Square inscribed in a Φ18mm circle
Image bit depth
12 bits
Compatible microscopes
Inverted microscope ECLIPSE Ti-E/Ti-U, Upright microscope ECLIPSE Ni-E (focusing nosepiece type/focusing stage type)/Ni-U, Fixed stage microscope ECLIPSE FN1, Multi-purpose zoom microscope AZ100
Z step
Ti-E: 0.025 um, FN1 stepping motor: 0.05 um, Ni-E: 0.025 um
Software
Software: NIS-Elements C
Display/image processing/analysis
2D/3D/4D analysis, time-lapse analysis, 3D volume rendering/orthogonal, spatial filters, image stitching, multipoint time-lapse, spectral unmixing, real-time unmixing, virtual filters, deconvolution, AVI image file output
Application: FRAP,FLIP, FRET, photoactivation, colocalization, three-dimensional time-lapse imaging, multipoint time-lapse imaging
Control Computer
OS: Microsoft Windows 7 Professional 64 bit SP1, CPU: Intel Xeon E5-1630v3 (3.70 GHz/10 MB/2133 MHz/Quad Core) or higher, Memory: 4 GB, Hard disk: Serial ATA 6 Gb/s (7,200 rpm) 146 GB or higher, Data transfer: LAN, Network interface: Gigabit Ethernet, Monitor: 1600 x 1200 or highter resolution, dual monitor, Configuration is recommended
Dimensions and weight
C2+/C2si+ scan head  145 (W) x 236.5 (H) x 70 (D) mm, Approx. 2 kg
Controller          225 (W) x 404 (H) x 382 (D) mm, Approx. 12 kg
Standard detector    225(W) x 154 (H) x 224 (D) mm, Approx. 5kg
Spectral detector     C2-DUS: 289 (W) x 323 (H) x 593.5 (D) mm, Approx. 23 kg,
                   C2-DUVB: 360 (W) x 91 (H) x 595.5 (D) mm, Approx. 9 kg
*1 The described frame rate is NOT available with Rotation, CROP, ROI, Spectral imaging and Stimulation.
*2 Only available with C2si+ scan head.
 
 


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