Photometrics Evolve™ 128 EMCCD Camera

優異的低亮度成像應用 

128 x 128 imaging array

24 x 24-μm pixels

Evolve™ 128擁有優異的性能,能供提供業界最低的 read noise,dark current,再加上electron multiplication (EM) gain,在低亮度的情況下,像是小動物活體成像到單分子螢光,都可以提供最好的Frames Rate,且透過eXcelon技術,可以進一步消除波紋,提高靈敏度。

  • 光電轉換效率大於90%
  • 128x128 imaging array
  • 24x24-um像素大小
  • 0.0069 e-/pixel/sec 暗電流
  • eXcelon獨家技術,可以進一步消除波紋,提高靈敏度
  • 在環境溫度20˚C 下,可以減至-85˚C
  • 滿井容量最高可達800,000e-
  • PAR(photometrics active regulation)回饋系統可以保證測量EM 值增益穩定性
  • FPGA設計,可以提供精確線性的EM gain,且有自動較正EM Gain功能,在長時間內保持定量狀態
  • ACE(Advanced Clocking Enhancement) Pixel-clock timing是其他em ccd的12倍,可以大幅減少背景信號以及雜電荷的產生
 

 

應用
InVivo Imaging ,
Calcium Imaging ,
Physiology,
Live Cell Microscopy ,
Single Molecule Fluorescence

 

Specifications
Read noise (e- rms @ Gain State 3)
 10 MHz EM Port 
46e- (Gain State 2)
Pixel Well Depth
Active area Gain register
200,000e-
800,000e-
Bias stability
A measurement of the camera stability when no light hits the sensor.
A slope of zero would be ideal. See footnote #1.
≤0.001 ADU/Frame
Gain stability
A measurement of the stability of the electron multiplication applied to images. Ideal value would be 0. The stability of the EM gain applied can be quantified by measuring the slope of a sequence of images with known amount of light and EM Gain applied. See footnote #2.
≤0.3 ADU/Frame (@ 10MHz, 350X, Gain State 2, 20K ADU)
Field uniformity
The image quality of the EMCCD is assessed for gradients. A complete lack of any gradient (i.e a flat image) would provide a numerical value of 1.00 See footnote #3.
10 MHZ EM                  1.07
Baseline bias value  500ADU but can be varied
Cooling temperature  Air cooled (@ ambient air 20˚C)                -85˚C
Dark current  0.0069 e-/pixel/sec (See footnote #4.)
Background events
 (10 MHz, 1000X EM gain) Standard operation
0.01 events/pixel
Relative charge transfer efficiency
Photometrics is able to measure this on each camera and optimize this parameter.
Optimized on each camera (See footnote #6.)
Charge transfer efficiency  As specified by CCD manufacturer’s data sheet (See footnote #7.)
Dark signal non-uniformity (DSNU) As specified by CCD manufacturer’s data sheet (See footnote #7.)
Photoresponse non-uniformity (PRNU)  As specified by CCD manufacturer’s data sheet (See footnote #7.)
Parallel shift rate  100 ns. This is optimized with Charge Transfer Efficiency (CTE) and CIC. Test results demonstrate that increasing parallel shift rate further can decrease CTE and increase CIC, adversely affecting image quality and sensitivity.

 



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